Demonstrations: Light/Dark Adaptation





1.  Rod vision. When you are reasonably dark adapted, dim lights are detected only by the rods, and bright lights are seen by both rods and cones. Here are several demonstrations of rod versus cone vision and dark adaptation.

(a) Close and tightly cover one eye (e.g. with you hand) for several minutes (at least 5, but preferably 10 minutes), while the other eye remains open and you are in a reasonably bright room. Next, turn the lights off (and cover all windows) so that the room is quite dim. Open your eyes one at a time and notice the huge difference in sensitivity between the two eyes (thus, dark adaptation is local to a given eye). Turn the lights back on and open your eyes one at a time. Note that the dark-adapted eye is dazzled (and takes time to "light adapt"), and the other eye is still reasonably light-adapted (because dark adaptation takes several minutes).

(b) Turn the lights off again, leaving very little illumination, and dark adapt for several minutes. Notice that you are color blind in regions that are the most dim, but that if objects are sufficiently bright, they appear as colored (this is the difference between rod and cone, or scotopic and photopic threshold).

(c)  In these dark-adapted conditions, find a very dim (dark) object on a darker background. Note that this distinction may not be visible if you look at it directly, but should be more visible if viewed slightly peripherally. This failure of foveal vision in the dark is a result of the rod-free area in the fovea (it is called "Arago's blind spot").

(d)  In these dark-adapted conditions, turn on a small light source (e.g. a penlight) and move it around. Note that you see trails of illumination which remain where the penlight has been. These trails are a result of the slowness of the rod system. The rods continue to respond with hyperpolarization even after the light landing on them has been extinguished. Of course, if the light lasts a reasonable amount of time concentrated on one area of the retina, then light adaptation will occur, and the spot which is still visible after the light is extinguished is an after-image. We can distinguish between trails and after-images by noticing that the trails occur even with very brief exposures to quite dim lights which are insufficient to cause appreciable light adaptation.