REQUIREMENT FOR mRNA SYNTHESIS DURING CONSOLIDATION
AND RECONSOLIDATION OF PAVLOVIAN AUDITORY FEAR CONDITIONING. |
S.Duvarci1*; K.Nader2; J.E.LeDoux1
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1. Ctr. Neural Sci, New York Univ, New York, NY, USA |
2. Psychology, McGill Univ, Montreal, PQ, Canada |
3. Ctr Neural Sci, New York Univ, New York, NY, USA |
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Synthesis of new RNA and proteins in the lateral-basal
amygdala (LBA) is necessary for the successful consolidation of new
auditory fear memories. Recently we have demonstrated that consolidated
auditory fear memories, when reactivated, return to a labile state
during which protein synthesis is again necessary for the reconsolidation
of the memory. Whether consolidation and reconsolidation are qualitatively
similar processes and are subject to same molecular mechanisms is
a question to be further examined. To this end, we tested the requirement
of mRNA synthesis in the LBA for the consolidation and reconsolidation
of auditory fear conditioning with the use of the mRNA synthesis inhibitor
DRB (5,6-Dichlorobenzimidazole 1-b-D-ribofuranoside). Rats were trained
with a single pairing of CS (5 KHz tone) and footshock US (1.5 mA,
1,5 sec). They were infused with either DRB or the vehicle immediately
after training and tested for short-term (STM) and the long-term memory
(LTM). To test for the requirement of mRNA synthesis during reconsolidation,
24 hr after training, memory was reactivated with one CS presentation.
Following reactivation, rats were infused with either DRB or vehicle
and were tested for post-reactivation STM and -LTM. The results showed
that both post-training and post-reactivation inhibition of mRNA synthesis
in the LBA impaired long-term, but not short-term, memory of the fear
conditioning.
Support Contributed By: the HFS, VW, to KN and JEL and NIMH to
JEL.
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Citation:
S. Duvarci, K. Nader, J.E. LeDoux. REQUIREMENT FOR mRNA SYNTHESIS
DURING CONSOLIDATION AND RECONSOLIDATION OF PAVLOVIAN AUDITORY FEAR
CONDITIONING. Program No. 623.10. 2003 Abstract Viewer/Itinerary
Planner. Washington, DC: Society for Neuroscience, 2003. Online.
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